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OVERVIEW OF
BIOCHEMICAL TECHNIQUE:

Biochemistry
involves the study of the chemical processes that takes place in living
organisms with which help to understand the nature of life in molecular terms. Biochemical
Studies rely on the availability of advancement of knowledge of the nature and
gives relationship between biological molecules  ,especially protein and nucleic acids and
cellular function.

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BIOCHEMICAL ANALYZERS:

·   Colorimeter

·   Spectrophotometer

·   Flame
Photometer

·   Auto
Analyzers

·   Blood
Gas Analyzers

·   Blood
Cell Counters

·   Hematology
Analyzers

·   Eliza
Reader

·   PCR Technique
Instruments

·   ESR
analyzers

·   Chromatography

·   Microscopy

Centrifugation

·    Electrophoresis

·   Radioisotopes

v SPECTROPHOTOMETRY:

It Deals with measurement of the radiant
energy transmitted or reflected by a body as a function of the wavelength.

Ø APPLICATION:

·       Used
to study the structure of compound.

·       Measurement
of Concentration.

·       Identification
Of unknown biomolecules

 

v COLORIMETRY:

It concerned with the measurement of
color compound. Its visible range is about 400-800nm.It is based on Beer- Lamberts Law.

Ø APPLICATIONS:

·      
It can be used to
find out the concentration of any colored compound 

·        measurements are made by using a light which passes through a
color filter

v FLAME PHOTOMETRY:

It deals with the quantitative
measurement of electrolytes.

e.g.: Sodium, potassium & lithium.

Ø APPLICATIONS:

·        
It is used for the determination of Alkali
and alkaline earth metals.

 

 

v ULTRACENTRIFUGATION:

It is used for the isolation of sub
cellular organelles or determination of molecular weight of Macromolecules

Ø APPLICATIONS:

·        
It is used for the separation of small
particles such as viruses

v RADIOIMMUNOASSAY:

RIA
is method used to identify small quantities of specific antibodies or antigens.

Ø APPLICATIONS:

·        
Radioimmunoassay is widely used for the
diagnosis of cancer and hormonal disorder.

·        
It is used for the therapeutic monitoring of
drugs.

·        
It is used for the biomedical research.

v ELIZA:

It is a  technique used mainly in immunology to detect the presence of an
antibody or an antigen in a sample.

Ø APPLICATIONS:

·   It
is used for the determination of small quantities of protein and other
biological substance.

·        
It is also been used for the diagnosis of
Aids.

v  POLYMERASE CHAIN REACTION:

PCR
is a cell-free amplification technique used to synthesize multiple identical copies
of DNA.

OR
This technique is used for generating large quantities of a specified DNA.

 

Ø APPLICATION:

·   PCR
is useful for the diagnosis of Inherited disease.

·   It
is used for the detection of bacterial injection .e.g Tuberculosis

·   This
technique is also used to detect Sex-linked disorder in fertilized embryos.

 

v CHROMATOGRAPHY:

It
is an analytical technique used for the separation of closely related compound
from a mixture

.

Ø  APPLICATIONS:

·        
Chromatography
is used in industrial processes to get rid of impurities chemicals,

·        
Chromatography
is the physical process by which complex mixtures are separated or analyzed.

v ELECTROPHORESIS:

Separation
of charged particles under the influence of electric forced is called
electrophoresis.

Ø  APPLICATIONS:

·   It
is used to separated biomolecules.(Protein)

It is also used for
identifying /purifying fra