involves the study of the chemical processes that takes place in living
organisms with which help to understand the nature of life in molecular terms. Biochemical
Studies rely on the availability of advancement of knowledge of the nature and
gives relationship between biological molecules ,especially protein and nucleic acids and
· PCR Technique
It Deals with measurement of the radiant
energy transmitted or reflected by a body as a function of the wavelength.
to study the structure of compound.
Of unknown biomolecules
It concerned with the measurement of
color compound. Its visible range is about 400-800nm.It is based on Beer- Lamberts Law.
It can be used to
find out the concentration of any colored compound
· measurements are made by using a light which passes through a
v FLAME PHOTOMETRY:
It deals with the quantitative
measurement of electrolytes.
e.g.: Sodium, potassium & lithium.
It is used for the determination of Alkali
and alkaline earth metals.
It is used for the isolation of sub
cellular organelles or determination of molecular weight of Macromolecules
It is used for the separation of small
particles such as viruses
is method used to identify small quantities of specific antibodies or antigens.
Radioimmunoassay is widely used for the
diagnosis of cancer and hormonal disorder.
It is used for the therapeutic monitoring of
It is used for the biomedical research.
It is a technique used mainly in immunology to detect the presence of an
antibody or an antigen in a sample.
is used for the determination of small quantities of protein and other
It is also been used for the diagnosis of
v POLYMERASE CHAIN REACTION:
is a cell-free amplification technique used to synthesize multiple identical copies
This technique is used for generating large quantities of a specified DNA.
is useful for the diagnosis of Inherited disease.
is used for the detection of bacterial injection .e.g Tuberculosis
technique is also used to detect Sex-linked disorder in fertilized embryos.
is an analytical technique used for the separation of closely related compound
from a mixture
is used in industrial processes to get rid of impurities chemicals,
is the physical process by which complex mixtures are separated or analyzed.
of charged particles under the influence of electric forced is called
is used to separated biomolecules.(Protein)
It is also used for
identifying /purifying fra